Laboratory of Lorraine S. Symington, Ph.D
Professor of Microbiology & Immunology

Selected Publications


Gnugge, R., Oh, J. and Symington, L.S. (2018) Processing of DNA double-strand breaks in yeast. Methods Enzymol. 600: 1-24.


Gnugge, R. and Symington, L.S. (2017) Keeping it real: MRX-Sae2 clipping of natural substrates. Genes Dev. 31: 2311-2312.


Ruff, P., Donnianni, R.A., Glancy, E., Oh, J. and Symington, L.S. (2016) RPA stabilization of single-stranded DNA is critical for break-induced replication. Cell Rep. 17: 3359-3368.


Ciccia, A. and Symington, L.S. (2016) Stressing out about RAD52. Mol. Cell 64: 1017-1019.


Symington, L.S. (2016) Mechanism and regulation of DNA end resection in eukaryotes. Crit. Rev. Biochem. Mol. Biol. 51: 195-212.


Oh, J., Al-Zain, A., Cannavo, E., Cejka, P. and Symington, L.S. (2016) Xrs2 dependent and independent functions of the Mre11-Rad50 complex. Mol. Cell 64: 405-415.


Deng, S.K., Yin, Y., Petes, T.D. and Symington, L.S. (2015) Mre11-Sae2 and RPA collaborate to prevent palindromic gene amplification. Mol. Cell 60: 500-508.


Chen, H., Donnianni, R.A., Handa, N., Deng, S.K., Oh, J., Timashev, L.A., Kowalczykowski, S.C. and Symington, L.S. (2015) Sae2 promotes DNA damage resistance by removing the Mre11-Rad50-Xrs2 complex from DNA and attenuating Rad53 signaling. Proc. Natl. Acad. Sci. U.S.A. 112: E1880-1887.


Deng, S.K., Chen, H. and Symington, L.S. (2015) Replication protein A prevents promiscuous annealing between short sequence homologies: Implications for genome integrity. Bioessays 37: 305-313.


Symington, L.S., Rothstein, R. and Lisby, M. (2014) Mechanisms and regulation of mitotic recombination in Saccharomyces cerevisiae. Genetics 198: 795-835.


Symington, L.S. (2014) DNA repair: Making the cut. Nature 514: 39-40.


Lee, A.H., Symington, L.S. and Fidock, D.A. (2014) DNA repair mechanisms and their biological roles in the malaria parasite Plasmodium falciparum. Microbiol Mol. Biol. Rev. 78: 469-486.


Deng, S.K., Gibb, B., Almeida, M.J., Greene, E.C. and Symington, L.S. (2014) RPA antagonizes microhomology-mediated repair of DNA double-strand breaks. Nature Struct. Mol. Biol. 21: 405-412.


Eissler, C.L., Mazon, G., Powers, B.L., Savinov, S.N., Symington, L.S. and Hall, M.C. (2014) The Cdk/Cdc14 module controls activation of the Yen1 Holliday junction resolvase to promote genome stability. Mol. Cell 54: 80-93.


Stafa, A., Donnianni, R.A., Timashev, L.A., Lam, A.F. and Symington, L.S. (2014) Template switching during break-induced replication is promoted by the Mph1 helicase in Saccharomyces cerevisiae. Genetics 196: 1017-1028.


Mazon, G. and Symington, L.S. (2013) Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination intermediates. Mol. Cell 52: 63-74.


Donnianni, R.A. and Symington, L.S. (2013) Break-induced replication occurs by conservative DNA synthesis. Proc. Natl. Acad. Sci. U.S.A. 110: 13475-13480.


Chen, H., Lisby, M. and Symington, L.S. (2013) RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol. Cell 50: 589-600.


Mazon, G., Lam, A.F., Ho, C.K., Kupiec, M. and Symington, L.S. (2012) The Rad1-Rad10 nuclease promotes chromosome translocations between dispersed repeats. Nature Struct. Mol. Biol. 9: 964-971.


Klein, H.L. and Symington, L.S. (2012) Sgs1-the maestro of recombination. Cell 149: 257-259.


Symington, L.S. and Gautier, J. (2011) Double-strand break end resection and repair pathway choice. Annu. Rev. Genet. 45: 247-271.


Mott, C. and Symington, L.S. (2011) RAD51-independent inverted-repeat recombination by a strand-annealing mechanism. DNA Repair 10: 408-415.


Mimitou, E.P. and Symington, L.S. (2011) DNA end resection-unraveling the tail. DNA Repair 10: 344-348.


Ho, C.K., Mazon, G., Lam, A.F. and Symington, L.S. (2010) Mus81 and Yen1 promote reciprocal exchange during mitotic recombination to maintain genome integrity in budding yeast. Mol. Cell 40: 988-1000.


Marrero, V.A. and Symington, L.S. (2010) Extensive DNA end processing by Exo1 and Sgs1 inhibits break-induced replication. PLoS Genetics 6: e1001007.


Mimitou, E.P. and Symington, L.S. (2010) Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2. EMBO Journal 29: 3358-3369.


Smith, C.E., Lam, A.F. and Symington, L.S. (2009) Aberrant double-strand break repair resulting in half crossovers in mutants defective for Rad51 or the DNA polymerase δ complex. Mol. Cell. Biol. 29: 1432-1441.


Fung, C.W., Mozlin, A.M. and Symington, L.S. (2009) Suppression of the double-strand break repair defect of the Saccharomyces cerevisiae rad57 mutant. Genetics 181: 1195-1206.


Mimitou, E.P. and Symington, L.S. (2008) Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing. Nature 455: 770-774.


Mozlin, A.M., Fung, C.W. and Symington, L.S. (2008) Role of the Saccharomyces cerevisiae Rad51 paralogs in sister-chromatid recombination. Genetics 178: 113-126.


Smith, C.E., Llorente, B and Symington, L.S. (2007) Template switching during break-induced replication. Nature 447: 102-105.