Department of Microbiology & Immunology

Columbia University in the City of New York
Saul J. Silverstein, Ph.D.
Professor of Microbiology & Immunology
Ph.D., University of Florida
Establishment, maintenance and reactivation from latency by varicella zoster virus

Research
The laboratory uses Varicella Zoster virus (VZV) to study virus latency. VZV is the causative agent of chicken pox (Varicella) and shingles (zoster). Following the productive infection that results in varicella the virus takes up residence in the neurons of the dorsal root ganglia and lays in wait to reactivate from latency.

We are particularly interested in deciphering the steps that lead to establishment of latency and reactivation of dormant virus from latency to cause zoster. During latency VZV expresses a number of transcripts that encode regulatory proteins and other proteins that are important for virus DNA replication. These proteins accumulate in the nucleus during lytic replication of the virus. However, in latently infected neurons they are predominantly found in the cytoplasm. We are interested in understanding how the neuron selectively partitions these proteins to the cytoplasm. Towards that end we have developed in vitro systems that recapitulate the VZV life cycle. We use these systems to identify host proteins that interact with and regulate protein localization.

Selected Publications

  1. Walters, M.S., Kinchington, P.R., Banfield, B.W. and Silverstein, S.J. (2010) Hyperphosphorylation of Histone Deacetylase 2 by Alphaherpesvirus US3 Kinases. J. Virol. 84: 9666-9676. pdf
  2. Walters, M.S., Kyratsous, C.A., and Silverstein, S.J. (2010) The RING Finger Domain of Varicella-Zoster Virus ORF61p has E3 Ubiquitin Ligase Activity That is Essential for Efficient Autoubiquitination and Dispersion of Sp100-Containing Nuclear Bodies. J. Virol. 84: 6861-6865. pdf
  3. Mueller, N.H., Walters, M.S., Marcus, R.A., Graf, L.L., Prenni, J., Gilden, D., Silverstein, S.J. and Cohrs, R.J. (2010) Identification of Phosphorylated Residues on Varicella-Zoster Virus Immediate-Early Protein ORF63 J. Gen. Virol. 91: 1133-1137. pdf
  4. Kyratsous, C.A., Walters, M.S., Panagiotidis, C.A. and Silverstein, S.J. (2009) Complementation of a Herpes Simplex Virus ICP0 null mutant by Varicella Zoster Virus ORF61p. J. Virol. 83: 10637-10643. pdf
  5. Walters, M.S., Erazo, A., Kinchington, P.R. and Silverstein, S.J. (2009) Histone Deacetylase 1 and 2 are Phosphorylated at Novel Sites During Varicella Zoster Virus Infection. J. Virol. 83: 11502-11513. pdf
  6. Kyratsous, C.A. and Silverstein, S.J. (2009) Components of Nuclear Domain 10 bodies Regulate Varicella Zoster Virus Replication. J. Virol. 83: 4262-4274. pdf
  7. Kyratsous, C.A., DeLong, C., Silverstein, S.J. and Panagiotidis, C.A. (2009) Chaperone-fusion Expression Plasmid Vectors for Improved Solubility of Recombinant Proteins in Escherichia coli. Gene 440: 9-15. pdf
  8. Kyratsous, C.A. and Silverstein, S.J. (2008) The co-chaperone BAG3 regulates Herpes Simplex Virus replication. Proc. Natl. Acad. Sci. U.S.A. 105: 20912-20917. pdf
  9. Walters, M.S., Kyratsous, C.A., Wan, S. and Silverstein, S.J. (2008) Nuclear Import of the Varicella Zoster Virus Latency-Associated Protein ORF63 in Primary Neurons Requires Expression of the Lytic Protein ORF61 and Occurs in a Proteasome-Dependent Manner. J. Virol. 82: 8673-8686.pdf
  10. Kyratsous, C.A. and Silverstein, S.J. (2007) BAG3, a Host Co-chaperone, Facilitates Varicella Zoster Virus Replication. J. Virol. 81: 7491-7503. pdf
  11. Storrs, C.H. and Silverstein, S.J. (2007) PATJ a Tight Junction-Associated PDZ Protein is a Novel Degradation Target of High-Risk HPV E6 and the Alternatively Spliced Isoform 18 E6*. J. Virol. 81: 4080-4090. pdf
  12. Stallings, C.L. and Silverstein, S.J. (2006) Posttranslational Modification and Cell-Type Specific Degradation of Varicella Zoster Virus ORF29p. J. Virol. 80: 10836-10846. pdf
  13. Schwartz, J.A., Brittle, E.E., Reynolds, A.E., Enquist, L.W. and Silverstein, S.J. (2006) UL54-Null Pseudorabies Virus Is Attenuated In Mice but Productively Infects Cells In Culture. J. Virol. 80: 769-784. pdf
  14. Stallings, C.L., Duigou, G.J., Gershon, A.A., Gershon, M.D. and S.J. Silverstein. (2006) The Cellular Localization Pattern of Varicella Zoster Virus ORF29p is Influenced by Proteasome-Mediated Degradation. J. Virol. 80: 1497-1512. pdf
  15. Stallings, C.L. and Silverstein, S.J. (2005) Dissection of a Novel Nuclear Localization Signal in ORF29 of Varicella Zoster Virus. J. Virol. 79: 13070-13081. pdf

Professor Saul J. Silverstein
Phone: 212-305-8149
Fax: 212-305-5106
Email:  sjs6@columbia.edu


Department of Microbiology & Immunology, Columbia University + 701 W. 168 St., HHSC 1208 New York, NY 10032 Tel. 212-305-3647