Department of Microbiology & Immunology

Columbia University Medical Center
Max E. Gottesman, M.D., Ph.D.
Charles H. Revson Professor of Biochemistry & Molecular Biophysics and Microbiology & Immunology
M.D., Ph.D., Yale University

Transcription termination in E. coli and bacteriophage, repair of double-strand breaks in eukaryotic DNA

Research
Our laboratory studies the regulation of transcription termination in E. coli and its bacteriophage. Based on the structures of the bacterial termination factors, we design mutations and determine their affects on termination. We are particularly interested in how transcription termination relates to translation and to DNA replication and chromosome integrity. We also study repair of DNA double-strand breaks in Xenopus oocyte extracts and in mammalian cells.

Selected Publications

  1. Bubunenko, M., Court, D.L., Al Refaii, A., Saxena, S., Korepanov, A., Friedman, D.I., Gottesman, M.E. and Alix, J.H. (2013) Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli. Mol. Microbiol. 87: 382-393.
  2. Williams, H.L., Gottesman, M.E. and Gautier, J. (2012) Replication-independent repair of DNA interstrand crosslinks. Mol. Cell. 47: 140-147.
  3. Dutta, D., Shatalin, K., Epshtein, V., Gottesman, M.E. and Nudler, E. (2011) Linking RNA polymerase backtracking to genome instability in E. coli. Cell 146: 533-543.
  4. Washburn, R.S. and Gottesman, M.E. (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108: 792-797.
  5. Burmann, B.M., Schweimer, K., Luo, X., Wahl, M.C., Stitt, B.L., Gottesman, M.E. and Rosch, P. (2010) A NusG:NusE complex links transcription and translation. Science 328: 501-504.
  6. Ben-Yehoyada, M., Wang L.C., Kozekov, I.D., Rizzo, C.J., Gottesman, M.E. and Gautier, J. (2009) Checkpoint signaling from a single DNA interstrand crosslink. Mol. Cell. 35: 704-15.
  7. Mooney, R.A., Schweimer, K., Roesch, P., Gottesman, M. and Landick, R. (2009) Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators. J. Mol. Biol. 391: 341-58.
  8. Luo, X., Hsiao, H-H., Bubunenko, M., Weber, G., Court, D.L., Gottesman, M.E., Urlaub, H. and Wahl, M.C. (2008) Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex. Mol. Cell 32: 791-802.
  9. Kim, H.C. and Gottesman, M.E. (2004) Transcription termination by phage HK022 Nun is facilitated by COOH-terminal lysine residues. J. Biol. Chem 279: 13412-13417.
  10. Feliciello, A., Gottesman, M.E., and Avvedimento, E.V. (2005) cAMP-PKA signaling to the mitochondria: protein scaffolds, mRNA and phosphatases. Cellular Signalling 17: 279-287
  11. Kim, H.C., Washburn, R.S, and Gottesman M.E. (2006) Role of E. coli NusA in Phage HK022 Nun-mediated Transcription Termination. J. Mol. Biol. 359: 10-12

Professor Max E. Gottesman
Phone: 212-305-6900
Lab Phone: 212-305-3705
Fax: 212-305-1741
Email:  meg8@columbia.edu


Department of Microbiology & Immunology, Columbia University + 701 W. 168 St., HHSC 1208 New York, NY 10032 Tel. 212-305-3647